What is Transcriptomics?
Transcriptomics is the study of the transcriptome, or all of the RNA molecules within a cell. Most studies of the transcriptome focus on the messenger RNA (mRNA) molecules only, which show the genes that are being actively expressed in a cell in a given environment or at a certain time. Methods for performing transcriptomics include RNA-Seq, microarrays and qRT-PR [1]
RNA-seqThe RNA-seq process. RNA fragments undergo reverse-transcription into cDNA fragments then adapters are added to the end of the fragments to allow for sequencing. Next Generation Sequencing is then used to create short sequences (or reads) that can then be aligned to a reference genome to produce a RNA sequence map. [2]
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MicroarraysThe Microarray process. An individual's DNA is compared to a control or "normal" sample by breaking up the single stranded DNA into smaller fragments and dying the control and subject's tissues two different colors. These dyed fragments are then applied to a plate containing DNA specific to the normal sequence of the gene. Any un-mutated fragments will bind to the cell while all mutated fragments will run off. [3]
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Transcriptomics and ARNT
Discussion
The data from these screens shows that ARNT shows very low specificity in he human body. ARNT plays a role in endothelial cell proliferation, so its presence in many areas of the body is expected. It is possible that though ARNT is regulated in many areas of the body, it has the most influential effect in the coronary artery of the heart.
References
[1] What is transcriptomics? (n.d.). Retrieved from https://www.phgfoundation.org/blog/what-is-transcriptomics
[2] RNA-seq: Basics, Applications and Protocol. (n.d.). Retrieved from https://www.technologynetworks.com/genomics/articles/rna-seq-basics-applications-and-protocol-299461
[3]DNA Microarray Technology Fact Sheet. (n.d.). Retrieved from https://www.genome.gov/about-genomics/fact-sheets/DNA-Microarray-Technology
[4] Basic Principles of RT-qPCR. (n.d.). Retrieved from https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/basic-principles-rt-qpcr.html
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[2] RNA-seq: Basics, Applications and Protocol. (n.d.). Retrieved from https://www.technologynetworks.com/genomics/articles/rna-seq-basics-applications-and-protocol-299461
[3]DNA Microarray Technology Fact Sheet. (n.d.). Retrieved from https://www.genome.gov/about-genomics/fact-sheets/DNA-Microarray-Technology
[4] Basic Principles of RT-qPCR. (n.d.). Retrieved from https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/basic-principles-rt-qpcr.html
Header image:https://www.mohrkeg.co.at/?service=cardiology
All other images are linked